The most basic substances of photosynthesis of green plants are chlorophyll, and their photosynthetic energy efficiency is affected by the amount of chlorophyll content, so the chlorophyll content is an important indicator of physiological changes such as plant nutritional status. The determination of chlorophyll content mainly includes spectrophotometry and SPAD502 chlorophyll meter method. The former is destructive, while the latter is a portable, SPAD502 chlorophyll meter has the characteristics of fast, convenient and non-destructive monitoring objects, and is often used to determine the relative content of chlorophyll in living leaves. The correlation between the chlorophyll content and the SPAD value and the mathematical model of Rhododendron amurensis leaves have not been reported yet. Therefore, the relationship between the measured value of chlorophyll and the SPAD value was analyzed to provide a reference for the prediction of the chlorophyll content using the SPAD502 chlorophyll meter in vivo.
Using the SPAD502 chlorophyll meter, the SPAD values ​​of the second and third leaves (representative of the main functional leaves) of the new shoot tip of the robust and disease-free plants were measured. Each leaf was taken from 6 different sites for determination, and finally taken. average value. The spectrophotometric method determines the absolute content of chlorophyll, while the SPAD502 chlorophyll meter measures the relative amount of chlorophyll, which can be called the leaf color value. Its working principle is to use two different wavelength light sources to illuminate the surface of the plant leaf, and the SPAD value is obtained by comparing the difference in the optical density of light transmitted through the leaf, which is a dimensionless ratio.
The SPAD502 chlorophyll meter was used to determine the relationship between SPAD value and chlorophyll content in the leaves of Fuling Jinling. The chlorophyll content showed an increasing trend with the increase of SPAD value. The best correlation function between chlorophyll a, chlorophyll b, chlorophyll a+b and SPAD values ​​was a power function model. The closeness of the correlation was chlorophyll a> chlorophyll a + b> chlorophyll b, which may be related to the SPAD chlorophyll analyzer. One of the working light sources is 660nm red, which is close to the maximum absorption peak wavelength of chlorophyll a (663nm), but related to the maximum absorption peak wavelength of chlorophyll b (645nm). Similar results are also found in other plants. .
Using the SPAD502 chlorophyll meter, the SPAD values ​​of the second and third leaves (representative of the main functional leaves) of the new shoot tip of the robust and disease-free plants were measured. Each leaf was taken from 6 different sites for determination, and finally taken. average value. The spectrophotometric method determines the absolute content of chlorophyll, while the SPAD502 chlorophyll meter measures the relative amount of chlorophyll, which can be called the leaf color value. Its working principle is to use two different wavelength light sources to illuminate the surface of the plant leaf, and the SPAD value is obtained by comparing the difference in the optical density of light transmitted through the leaf, which is a dimensionless ratio.
The SPAD502 chlorophyll meter was used to determine the relationship between SPAD value and chlorophyll content in the leaves of Fuling Jinling. The chlorophyll content showed an increasing trend with the increase of SPAD value. The best correlation function between chlorophyll a, chlorophyll b, chlorophyll a+b and SPAD values ​​was a power function model. The closeness of the correlation was chlorophyll a> chlorophyll a + b> chlorophyll b, which may be related to the SPAD chlorophyll analyzer. One of the working light sources is 660nm red, which is close to the maximum absorption peak wavelength of chlorophyll a (663nm), but related to the maximum absorption peak wavelength of chlorophyll b (645nm). Similar results are also found in other plants. .
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