6 Ways to Study Cotton Roots
1 Mining method
Mining method is one of the most traditional and common methods. It can be divided into complete mining method and two-way slice method. The complete excavation method is suitable for the use when the cotton root system has a small distribution range of roots. In the specific application, there are different methods such as direct excavation method, potted method, metal frame method, and plastic mesh bag method.
2 Root underground observation room method
This method can be directly observed by observing the glass root growth observation box in the room. Its greatest advantage is that it can continuously observe the growth of the root system without destroying the root system. This is unmatched by other methods. However, the establishment of a root underground observation room is too expensive, and the calculation of the length of the root system is relatively time-consuming and cannot be widely adopted by the majority of scientific research workers.
3 root drilling
The root drilling method uses a human and mechanical method to remove the root soil sample from the field and wash out the root system. This method is currently the most widely used and ideal method for measuring the density of roots in a unit soil. Its main advantage is that the sampling speed in the field is fast, it is conducive to set up multiple repetitions, the degree of damage to the soil is light, and the distribution of the root system in different soil layers can also be analyzed. However, for a crop with a lower root density, errors may occur due to the small diameter of the soil column, and it is not suitable for root morphological studies.
4 container method
The container method is mainly used for morphological, physiological and biochemical research of plant roots, and also for root ecology research. Using container methods combined with different treatment factors for research, a large amount of knowledge about the relationship between root growth and influencing factors can be obtained.
5 Indirect method
Indirect methods include staining and trace element methods. The dyeing method is to inject a colored solution on the stem of the plant, and then observe the distribution of the liquid in the root system; Trace elements include radioactive and non-radioactive, and the radioactive element tracer method is used in determining the root activity of the crop. The advantage of the element tracer method is that it can achieve the purpose of research without separating the roots and soils, and has the advantages of saving time and labor. But generally it can only be used for supporting research.
6 Application of Modern Root Analysis System
The basic principle of the modern root analysis system is to combine the scanning system with graphic analysis software. The image analysis software is used to analyze the scanned root image, and the root length, surface area, volume, and root coefficient amount are calculated. At present, the domestically used GXY-A root analysis system, which is manufactured by Zhejiang Top Instrument, is relatively mature.
Diamond Polishing Pad,Diamond Stone Polishing Pad,Diamond Concrete Polishing Pad,Quality Diamond Polishing Pad
Henan Huanghe Whirlwind International Co., Ltd. , https://www.huangheindustry.com