[China Instrument Network Instrument R&D] Recently, the specialization project of quality inspection public welfare industry undertaken by the Biometrics Institute of the Institute of Metrology and Measurement Technology, Shanghai Research Institute of Molecular Biology Measurement Related to Bacteria Detection (project number) Successfully passed expert acceptance.
The “Research on Molecular Biology Measurement Techniques Related to Bacteria Detection†project aims to solve the problem of traceability of pathogenic microorganisms detected by molecular biological detection techniques. 19 kinds of plasmid DNA reference materials were developed to cover common pathogenic microorganisms, including: Species conserved sequences of microorganisms such as Enterobacter sakazakii, Salmonella, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Bacillus anthracis, Vibrio cholerae, Mycobacterium tuberculosis, and key DNA sequences of important drug-resistant bacteria; ATP was developed Purity standard substance and solution standard substance; Completion of a variety of nucleic acid valuation analysis methods including high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS), digital PCR (dPCR), etc.; based on self-developed standards Substances, standards, and a variety of microbial molecular biological detection instrument traceability methods. The project team has extensively carried out calibration and calibration services for PCR analyzers, micronuclear protein quantifiers, and chemiluminescence analyzers using the results of the above projects, and achieved significant social and economic benefits.
Through technical breakthroughs in nucleic acid quantitative analysis methods and standard substance research, this project has overcome the technical difficulties in traceability of nucleic acid quantitative processes, and formed a complete traceability system for quantitative genetic measurements, providing an effective measurement basis for genetic testing-related technical fields. Support has important practical application value.
(Original title: "Research on molecular biology traceability technology related to bacterial detection" project passed acceptance)
The “Research on Molecular Biology Measurement Techniques Related to Bacteria Detection†project aims to solve the problem of traceability of pathogenic microorganisms detected by molecular biological detection techniques. 19 kinds of plasmid DNA reference materials were developed to cover common pathogenic microorganisms, including: Species conserved sequences of microorganisms such as Enterobacter sakazakii, Salmonella, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Bacillus anthracis, Vibrio cholerae, Mycobacterium tuberculosis, and key DNA sequences of important drug-resistant bacteria; ATP was developed Purity standard substance and solution standard substance; Completion of a variety of nucleic acid valuation analysis methods including high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS), digital PCR (dPCR), etc.; based on self-developed standards Substances, standards, and a variety of microbial molecular biological detection instrument traceability methods. The project team has extensively carried out calibration and calibration services for PCR analyzers, micronuclear protein quantifiers, and chemiluminescence analyzers using the results of the above projects, and achieved significant social and economic benefits.
Through technical breakthroughs in nucleic acid quantitative analysis methods and standard substance research, this project has overcome the technical difficulties in traceability of nucleic acid quantitative processes, and formed a complete traceability system for quantitative genetic measurements, providing an effective measurement basis for genetic testing-related technical fields. Support has important practical application value.
(Original title: "Research on molecular biology traceability technology related to bacterial detection" project passed acceptance)